Insert contains SacI (3), SphI (1), and HincII (1) sites. Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.1; HincII--4.2, plus smaller; HindIII--4.4; PstI--4.4; BamHI--4.4. The protein resulting from in vitro translation of this cDNA (unglycosylated, with signal peptide) is precipitated by MAb 17D6 specific to Blast-1. Detects human genomic restriction fragments of the following sizes (kb): EcoRI--9.3, 6.7, 3.4 (weak); HindIII--2.9, 2.1, 1.2, 0.8. This is a full-length or close to full-length cDNA clone including 46 bp 5' untranslated and about 430 bp 3' untranslated sequence. The sequence predicts 5 N-glycosylation sites. The insert is oriented such that transcription from the T7 promoter gives the sense strand. |
