Insert contains HindIII, SstI, and 2 XbaI sites. Restriction digests of the clone give the following sizes (kb): BamHI--7.1; AvaI--7.1; HindIII--4.5, 2.6; PstI--4.5, 1.8, 0.8; EcoRI--4.5, 2.6. Cross-hybridizes with chicken myb sequences. EcoRI RFLP demonstrated co-dominant segregation in 1 informative family of 9. Allele A1 is amplified in ML3 and COLO 210 cell lines. Enzyme(s) not detecting polymorphism: HindIII, BglII, BamHI, SstI, PstI. Tested against 5 unrelated individuals. |
