| 產(chǎn)品名稱 | Mfn1/Mfn2-null MEFs |
|---|---|
| 商品貨號 | B161974 |
| Organism | Mus musculus, mouse |
| Tissue | embryo/embryo fibroblast |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast-like |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain SV40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | embryo, 10.5-days gestation |
| Applications | These cell lines are useful in studying mitochondrial biology and the role of mitochondrial fusion in cell physiology. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | MEFs were derived from embryos 10.5 days gestation. Embryos were mechanically dispersed by repeated passage through a P1000 pipette tip and plated with MEF medium (DME, 10% FCS, 1X nonessential amino acids, 1 mM L-glutamine (Invitrogen/Gibco)). Cells with deletions of Mfn1 (ATCC CRL-2992) or Mfn2 (ATCC CRL-2993) or both Mfn1/Mfn2-null MEFs (ATCC CRL-2994) were subsequently immortalized by transduction with SV-40 T antigen. |
| Clinical Data | Donor Organism Characteristics: knockout |
| Comments | Both Mfn1 and Mfn2 are essential for mitochondrial fusion which is essential for embryonic development. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:5 to 1:20 is recommended Medium renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: complete growth medium, 90%; DMSO, 10% liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor | D Chan |
| Year of Origin | 2003 |
| References | Chen H, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 160: 189-200, 2003. PubMed: 12527753 Koshiba T, et al. Structural basis of mitochondrial tethering by mitofusin complexes. Science 305(5685): 858-862, 2004. PubMed: 15297672 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
