| 產品名稱 |
311-3D4 |
| 商品貨號 |
B161864 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension, but many cells attach |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with a synthetic polypeptide with the sequence LWEIATYGMSPYPGIDLSQVY(C) corresponding to peptides 429 to 449 of the abl oncogene (formerly 548 to 568 of the v-abl oncogene). In ELISA testing, the antibody reacts with the abl sequence. No binding activity was detected for the homologous region of FES, v-erb B, v-src, SRC, v-yes, RAF-1, v-raf, fms, v-fps, v-ros, HIR, v-mos, and SYN. In immunoblot testing a protein of approximately 40 kd is detected in ANN-1 and src transformed cells. |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide |
| Comments |
Animals were immunized with a synthetic polypeptide with the sequence LWEIATYGMSPYPGIDLSQVY(C) corresponding to peptides 429 to 449 of the abl oncogene (formerly 548 to 568 of the v-abl oncogene). Spleen cells were fused with Sp2/0 myeloma cells. In ELISA testing, the antibody reacts with the abl sequence. No binding activity was detected for the homologous region of FES, v-erb B, v-src, SRC, v-yes, RAF-1, v-raf, fms, v-fps, v-ros, HIR, v-mos, and SYN. In immunoblot testing a protein of approximately 40 kd is detected in ANN-1 and src transformed cells. The IgG1 reactivity of the antibody with the ABL peptide (429 to 449) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Scrape off the attached cells and transfer along with the floating cells into new flasks. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 and IgG2b |
| Antigenic Determinant |
abl oncogene peptide, synthetic |
| Name of Depositor |
National Cancer Institute |
| Deposited As |
mouse (B cell); mouse (myeloma) |
